acs 5004 Search Results


neural progenitor cells  (ATCC)
93
ATCC neural progenitor cells
Human Neural <t>Progenitor</t> Cells (NPCs) display increased neurite outgrowth when cultured with exosomes and CM from MCF-7 Snail cells compared to MCF-7 Neo. MCF-7 Neo or MCF-7 Snail was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. (B) The percentage of NPCs with neurite outgrowth and average neuron length was measured utilizing Image J Fiji Just software in NPCs cultured with NPC medium, Exo-D medium, and Exo-D medium plus NGF-beta as a positive control. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (C) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (D) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, ***p< 0.001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.
Neural Progenitor Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acs+5004/Neural+Progenitor+Cells+Derived+from+ATCC-BYS012+Normal%3B+Human/pmc12825424-54-0-7
Average 93 stars, based on 1 article reviews
neural progenitor cells - by Bioz Stars, 2026-09
93/100 stars
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bys012 atcc acs  (ATCC)
94
ATCC bys012 atcc acs
Human Neural <t>Progenitor</t> Cells (NPCs) display increased neurite outgrowth when cultured with exosomes and CM from MCF-7 Snail cells compared to MCF-7 Neo. MCF-7 Neo or MCF-7 Snail was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. (B) The percentage of NPCs with neurite outgrowth and average neuron length was measured utilizing Image J Fiji Just software in NPCs cultured with NPC medium, Exo-D medium, and Exo-D medium plus NGF-beta as a positive control. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (C) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (D) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, ***p< 0.001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.
Bys012 Atcc Acs, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acs+5004/Neural+Progenitor+Cells+Derived+from+ATCC-BYS0112+Normal%3B+Human/pm37494934-209-88-89
Average 94 stars, based on 1 article reviews
bys012 atcc acs - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier

Image Search Results


Human Neural Progenitor Cells (NPCs) display increased neurite outgrowth when cultured with exosomes and CM from MCF-7 Snail cells compared to MCF-7 Neo. MCF-7 Neo or MCF-7 Snail was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. (B) The percentage of NPCs with neurite outgrowth and average neuron length was measured utilizing Image J Fiji Just software in NPCs cultured with NPC medium, Exo-D medium, and Exo-D medium plus NGF-beta as a positive control. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (C) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (D) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, ***p< 0.001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.

Journal: Journal of Cancer

Article Title: Talin1 Mediates Tumor-Nerve Interactions in Prostate and Breast Cancer Cells

doi: 10.7150/jca.127292

Figure Lengend Snippet: Human Neural Progenitor Cells (NPCs) display increased neurite outgrowth when cultured with exosomes and CM from MCF-7 Snail cells compared to MCF-7 Neo. MCF-7 Neo or MCF-7 Snail was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. (B) The percentage of NPCs with neurite outgrowth and average neuron length was measured utilizing Image J Fiji Just software in NPCs cultured with NPC medium, Exo-D medium, and Exo-D medium plus NGF-beta as a positive control. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (C) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (D) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, ***p< 0.001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.

Article Snippet: Neural Progenitor Cells (ACS-5004), NPC Growth Medium (ATCC ACS-3003), DMEM: F12 (ATCC 30-2006) and Cell Basement Membrane (ACS-3035) were purchased from American Tissue Cell Culture (ATCC), Manassas, VA. Talin1 inhibitor, mH4 and F3 protease inhibitor control, was obtained from Dr. Kensei Tsuzaka, Kaytee Bio, Co. & Ltd., Chiba, Japan. mH4 was dissolved in DMSO to a stock solution of 14 mg/ml.

Techniques: Cell Culture, Isolation, Incubation, Fluorescence, Microscopy, Software, Positive Control

Human neural progenitor cells (NPCs) display reduced neurite outgrowth with exosomes and CM from C4-2 Snail knockdown (C4-2 E8) cells compared to C4-2 Non-silencing (NS) cells. C4-2 NS or C4-2 E8 was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes, or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (B) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (C) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.

Journal: Journal of Cancer

Article Title: Talin1 Mediates Tumor-Nerve Interactions in Prostate and Breast Cancer Cells

doi: 10.7150/jca.127292

Figure Lengend Snippet: Human neural progenitor cells (NPCs) display reduced neurite outgrowth with exosomes and CM from C4-2 Snail knockdown (C4-2 E8) cells compared to C4-2 Non-silencing (NS) cells. C4-2 NS or C4-2 E8 was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes, or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (B) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (C) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.

Article Snippet: Neural Progenitor Cells (ACS-5004), NPC Growth Medium (ATCC ACS-3003), DMEM: F12 (ATCC 30-2006) and Cell Basement Membrane (ACS-3035) were purchased from American Tissue Cell Culture (ATCC), Manassas, VA. Talin1 inhibitor, mH4 and F3 protease inhibitor control, was obtained from Dr. Kensei Tsuzaka, Kaytee Bio, Co. & Ltd., Chiba, Japan. mH4 was dissolved in DMSO to a stock solution of 14 mg/ml.

Techniques: Knockdown, Cell Culture, Isolation, Incubation, Fluorescence, Microscopy, Software